Description
R-Phycoerythrin is a vibrant red fluorescent protein commonly used in various scientific and biotechnological applications. Derived primarily from red algae, it serves as an accessory pigment in photosynthesis, aiding in the absorption of light, particularly in the blue-green to yellow-orange spectrum. This protein is highly prized in flow cytometry and fluorescence microscopy for its bright fluorescence and high quantum yield, which facilitate the visualization and analysis of cellular and molecular processes. Furthermore, R-phycoerythrin's ability to conjugate with antibodies or other biomolecules makes it an invaluable tool in immunology and cell biology, enhancing signal detection and enabling detailed study of complex biological systems. Its stability and intense fluorescence properties continue to make R-phycoerythrin a preferred choice in advanced analytical techniques.
Specification
Item | R-Phycoerythrin |
---|
Catalog | MC11016174 |
CAS | 11016-17-4 |
Molecular Weight | 240 kDa |
Form | Liquid |
Color | Brown to reddish brown |
MDL | MFCD00081950 |
InChI | 1S/C10H16N5O13P3/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20)/t4-,6-,7-,10-/m1/s1 |
InChI Key | ZKHQWZAMYRWXGA-KQYNXXCUSA-N |
Canonical SMILES | C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N |
Isomeric SMILES | C1=NC(=C2C(=N1)N(C=N2)[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N |
Excitation | 565 nm |
Emission | 576 nm |
Formulation | Supplied in phosphate buffered saline |
Concentration | 12-20 mg/ml |
Storage | Store in the dark at 2-8 °C. Avoid prolonged exposure to light. |
Properties
R-Phycoerythrin has a range of properties that make it a valuable tool in many areas of biological research, particularly in techniques that require labeling and detecting specific biomolecules or cells. Here are some of its key properties:
- Fluorescence property. R-Phycoerythrin is widely used as a fluorescent dye due to its high quantum yield and large Stokes shift. It emits fluorescence in the orange/red spectrum when excited by light of shorter wavelengths.
- Molecular structure. R-Phycoerythrin is composed of a protein complex with chromophores known as phycobilins, which are responsible for its color and fluorescent properties. It consists of alpha and beta subunits.
- Stability. R-Phycoerythrin is generally stable under a range of conditions, but it is sensitive to light, pH changes, and high temperatures, which can affect its fluorescence.
Choose Alfa Chemistry
With a strong commitment to quality and a relentless pursuit of innovation, Alfa Chemistry strives to unleash the unlimited potential of marine resources and provide a diverse range of quality products. We have the ability to provide you with high-quality R-phycoerythrin at a competitive price. If you have any requirements, please contact us immediately.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.